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. Author manuscript; available in PMC: 2010 Feb 11.
Published in final edited form as: J Immunol. 2009 May 1;182(9):5363. doi: 10.4049/jimmunol.0803980

Figure 2.

Figure 2

Restoration of IR injury in Rag1−/− mice by mAb B4. Sixty min prior to the induction of ischemia by mesenteric artery occlusion, 25 μg of purified mAb B4, or 100 μg of control IgM mAb D5, was injected i.v. Following completion of the IR protocol, mice were sacrificed and Giemsa-stained intestinal sections from each treatment group were scored for mucosal injury (0–6) as described in Materials and Methods. Monoclonal Ab B4 injected into Rag1−/− mice induced substantial injury in the mice undergoing IR (mAb B4 IR) in contrast to sham operated mice (Rag1−/− sham) or Rag1−/− mice undergoing IR (Rag1−/− IR). MAb D5 treated Rag1−/− mice (mAb D5 IR) did not demonstrate substantial injury. As a positive control for the level of intestinal injury, wildtype C57Bl/6 mice undergoing IR were included (wt IR). All measurements were obtained at x200 magnification. The figure is a representative of two independent experiments. Each bar is the average ± SEM with 3–6 animals per group. Statistical analysis was performed by Student’s t test.