Figure 1.

Effect of bacterial growth on the rate of degradation of GSNO in DMEM. Log phase wild-type N. meningitidis (squares; dotted line) or the norB-mutant derivative (triangles; broken line) were grown in DMEM + 10% HI-FCS supplemented with 100 μM GSNO (circles; solid line). SNO concentration was determined at intervals by duplicate injection of acidified sulfanilamide-treated medium into I₃⁻ reaction mixture and quantified by ozone-based chemiluminescence. Data are percentages of the starting SNO concentration. Individual points, representing means + se, were compared using unpaired samples t test. Area under curve for the three groups was compared by unpaired samples t test. n > 5. Error bars fall within the points where not visible.