Figure 4.

shRNA-mediated repression of PAFr in Caco-2 monolayers reduces translocation of E. faecalis. (A) PAFr is not induced by hypoxia in PAFr knockdown Caco-2 monolayers incubated in hypoxia. (B) Loss of protein induction by PAFr knockdown Caco-2 monolayers after 24 h in hypoxia. (C) Translocation of E. faecalis across hypoxic and normoxic PAFr knockdown Caco-2 monolayers. CV-6209 (60 μM) does not reduced translocation across PAFr knockdown Caco-2 monolayers. Bacterial translocation was over 120 min. Results are derived from six experiments. Hx, hypoxia; Nx, normoxia. **p < 0.01 and ***p < 0.005 (Student's t test).