Table 1.
Candidate kinases that phosphorylate 12E8 tau
| 12E8 Tau/Control | Total Tau | 12E8 Tau/Total Tau | ||||||||
| Gene Name | Cell Count | Average | St Dev | p value | Average | St Dev | p value | Average | St Dev | p value |
| MARK2 | 453 | 0.672 | 0.044 | 0.006 | 0.979 | 0.039 | 0.451 | 0.687 | 0.060 | 0.012 |
| PAK3 | 170 | 0.803 | 0.067 | 0.036 | 1.112 | 0.197 | 0.429 | 0.730 | 0.072 | 0.023 |
| PAK2 | 287 | 0.726 | 0.081 | 0.028 | 0.986 | 0.101 | 0.831 | 0.741 | 0.109 | 0.054 |
| ADCK5 | 280 | 0.716 | 0.104 | 0.042 | 0.934 | 0.094 | 0.351 | 0.765 | 0.069 | 0.028 |
| AKAP13 | 181 | 0.738 | 0.069 | 0.023 | 0.929 | 0.070 | 0.223 | 0.797 | 0.086 | 0.054 |
| LOC55971 | 152 | 0.836 | 0.035 | 0.014 | 0.982 | 0.019 | 0.241 | 0.852 | 0.042 | 0.026 |
| PLK2 | 335 | 0.774 | 0.087 | 0.046 | 0.906 | 0.114 | 0.289 | 0.856 | 0.052 | 0.040 |
| DYRK1A | 316 | 0.774 | 0.051 | 0.017 | 0.848 | 0.104 | 0.127 | 0.860 | 0.038 | 0.023 |
| MAK | 393 | 1.109 | 0.021 | 0.012 | 0.919 | 0.037 | 0.063 | 1.209 | 0.054 | 0.021 |
| ITK | 285 | 1.124 | 0.036 | 0.027 | 0.887 | 0.059 | 0.079 | 1.273 | 0.118 | 0.057 |
| PIM1 | 409 | 1.248 | 0.097 | 0.047 | 0.965 | 0.105 | 0.624 | 1.297 | 0.080 | 0.023 |
| RAGE | 397 | 1.305 | 0.106 | 0.038 | 0.960 | 0.088 | 0.511 | 1.361 | 0.053 | 0.007 |
| ITPK1 | 399 | 1.324 | 0.119 | 0.042 | 0.930 | 0.084 | 0.285 | 1.424 | 0.024 | 0.001 |
| CKB | 223 | 1.537 | 0.143 | 0.023 | 1.065 | 0.146 | 0.523 | 1.454 | 0.138 | 0.030 |
| PFKM | 375 | 1.305 | 0.096 | 0.032 | 0.888 | 0.112 | 0.227 | 1.476 | 0.082 | 0.010 |
| DGKB | 440 | 1.350 | 0.096 | 0.024 | 0.921 | 0.163 | 0.488 | 1.489 | 0.200 | 0.052 |
| SPHK2 | 333 | 1.792 | 0.282 | 0.040 | 1.074 | 0.121 | 0.399 | 1.664 | 0.114 | 0.010 |
A list of kinases affecting 12E8 tau phosphorylation. Shown are the most significant effectors of 12E8 tau phosphoylration emerging from a high-content siRNA screen of the complete "kinome." Indicated in the columns from left to right are, the Gene Name, the cell counts (which refers to the number of cells in the well following transfection and is and indicator of potential siRNA toxicity), and then the average fold effect, standard deviation, and p value for 12E8 tau, total tau, and the ratio of 12E8 tau/total tau. For a reference on cell counts, non-siliencing s iRNA control cell counts range from ~330-380, depending on the individual plates in the assay. This tabel contains only those siRNAs that affect pS262 tau/total tau through significant effects on pS262 tau alone. Total tau levels were selected to be insignificantly effected (p > 0.05), although some candidates clearly show a strong trend toward significant reductions in total tau.