Table 2.
Hour | Category | Control | 25 mM Furfural | 50 mM Furfural |
---|---|---|---|---|
6 | Single/large | 86.5 ± 6 | 48 ± 8 | 30.0 ± 3 |
2-4/Medium | 8.5 ± 1 | 22 ± 4 | 35.5 ± 4 | |
> 4/Small | 5.0 ± 5 | 30 ± 12 | 34.5 ± 6 | |
24 | Single/large | 77 ± 13 | 36 ± 16 | 7.5 ± 8 |
2-4/Medium | 16 ± 6 | 21 ± 0.2 | 18.0 ± 13 | |
> 4/Small | 7 ± 8 | 43 ± 16 | 74.5 ± 21 | |
48 | Single/large | 79.5 ± 1 | 28 ± 5 | 1 ± 1 |
2-4/Medium | 15.5 ± 4 | 22 ± 5 | 7 ± 3 | |
> 4/Small | 5.0 ± 3 | 49 ± 7 | 92 ± 4 |
Prior to adding furfural, 96%, 2% and 2% of cells contained large, medium and small shaped vacuoles, respectively. Data represent averages of two experiments with standard error indicated. At least 100 cells were examined at each time point.