Table 2. Kinetic Parameters for HBV Polymerases.
| dGTP Km, nMa (Fold WT)b | Ki, nM (Fold WT) | Ki/Km (Fold WT) | kcat, min−1 (Fold WT) | |
| Wildtype (WT) | 5.0±0.5 (1) | 0.4 (1) | 0.08 (1) | 0.24 (1) |
| LVDr M204V+L180M | 3.5±0.4 (14) | 12.3 (30.8) | 3.5 (43.8) | 0.093 (2.58) |
| LVDr + T184L | 1.9±0.6 (2.6) | 224.5 (561.3) | 118.2 (1477.5) | 0.13 (1.85) |
| LVDr + S202G | 2.1±0.1 (2.4) | 146.7 (366.8) | 69.9 (873.8) | 0.11 (2.18) |
| LVDr + M250V | 2.3±0.5 (2.2) | 121.2 (303.0) | 52.7 (658.8) | 0.17 (1.41) |
| LVDr + T184G + S202I | 0.7±0.1 (7.1) | 155.1 (387.8) | 221.6 (2770.0) | 0.076 (3.16) |
a
Values are the mean ± standard deviation from at least three independent experiments.
b
Fold change from wildtype value.