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. 2010 Feb 12;5(2):e9197. doi: 10.1371/journal.pone.0009197

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Gwinn et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Raptor undergoes a mobility shift on SDS-PAGE following nocadazole which is collapsed by phosphatase treatment. Myc-tagged raptor was expressed in HEK293T cells and nocadazole treated for 16 h. Where indicated, immunoprecipitates were treated with or without calf-intestinal alkaline phosphatase (CIP) and then resolved in SDS-PAGE, and subjected to anti-myc immunoblotting. (B) Raptor is recognized by a phospho-threonine proline antibody in mitotic arrested cells. HEK293T cells transiently expressing myc-tagged raptor were treated for 16 h with taxol and immunoprecipitates were immunoblotted with an antibody that recognizes phospho-threonine followed by proline.