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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Gene Ther. 2009 Oct 22;17(2):217–226. doi: 10.1038/gt.2009.136

Figure 1. Localization of φC31 integrase with and without a NLS in HeLa cells.

Figure 1

The φC31 integrase was fused to the 9 amino acid HA sequence to permit visualization, as well as an NLS before or after the HA sequence. HeLa cells grown on coverslips were transfected with the constructs indicated. The cells were fixed with 4% paraformaldehyde and stained for the HA tag and with Hoechst to label the DNA. Inset is a 9-fold amplification of the merge image. The NLS effectively relocalized the protein from the cytoplasm to the nucleus.