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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Glia. 2010 Apr;58(5):611–621. doi: 10.1002/glia.20949

Figure 4.

Figure 4

Jnk and Erk1/2 signaling pathways, but not p38, are affected by apocynin treatment in stimulated U-87 astrocytes. (A) Western Blot analysis of cytosolic lysates collected from cells untreated, treated with Tat/IFN-γ/TNF-α, or pre-treated with apocynin prior to stimulation with Tat/IFN-γ/TNF-α for 60 min. The blots were probed with antibodies against phospho-p38, phospho-Jnk, and phospho-Akt. (B, C and D) Densitometric scans illustrating the ratio of phospho-Jnk, Erk1/2 and p38 normalized to β-actin levels. Data are representative of one of three replicate experiments. **p<0.01; ***p<0.001 vs control group; #p<0.05, ##p<0.01 vs Tat/IFN-γ/TNF-α group.