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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2010 Jan 26;9(2):510. doi: 10.1158/1535-7163.MCT-09-0461

Fig. 2.

Fig. 2

Fig. 2

Fig. 2

Fig. 2

Melanoma cell lines signal via the Jak-STAT pathway. (A) The human melanoma cell lines 1106 MEL, A375, F01, and 1174 MEL cells were treated with IFN-α at 104 units/ml or IL-29 at 10, 100, or 1000 ng/ml and evaluated by immunoblot analysis for P-STAT1/2/3/5. Membranes were probed with β-actin antibody as a loading control. Human melanoma cell lines 1106 MEL, A375, F01, and 1174 MEL were stimulated with increasing doses of IL-29 (0–1000 ng/ml) for 30 minutes at 37°C and cells were evaluated for P-STAT1 by intracellular flow cytometry. Fluorescence data are presented as Fsp intensity of P-STAT1 staining (Fsp=Ft−Fb). (B) Representative histograms for a single experiment and (C) graph for all cell lines evaluated. Columns; mean from a single experiment, bars; SD. (D) Protein levels of pAKT, pERK, and pSAPK were measured by immunoblot analysis after no treatment (0), or treatment with PMA (50 ng/mL), or IL-29 (10, 100, 1000 ng/mL).