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. Author manuscript; available in PMC: 2011 Feb 2.
Published in final edited form as: Mol Cancer Ther. 2010 Feb 2;9(2):438. doi: 10.1158/1535-7163.MCT-09-0682

Figure 2. Mutant template telomerase RNA (MT-mTer) incorporates into active telomerase enzyme and reprograms it to add mutated telomeric repeats.

Figure 2

(A) MT-mTer and MT-mTer/siRNA structure and cloning into lentiviral vector system. (B) Ectopic over-expression (50 fold) of MT-mTer in E4 mouse prostate cancer cells three days after lentiviral infection, as quantified by RT-PCR. (C) MT-mTer levels by PCR are unaffected by co-expresseed α-mTer-siRNA, which is designed to knock down only the endogenous wild type mTer. (D) Experiment scheme of modified RT-PCR TRAP assay designed to specifically detect addition of wild-type TTAGGG vs. mutated TTTGGG nucleotide repeats.