Table 1. Mutant template telomerase RNA (MT-mTer) reprograms telomerase enzyme to add mutated telomeric repeats.

When MT-mTer and MT-mTer/siRNA are over-expressed, significantly more telomeric products (270% and 350%, respectively) are detected using a TTTGGG-specific (“4A10A”) reverse primer in the second (amplification) step of RT-PCR TRAP, an effect which is preserved even when just dGTP and dTTP are added in the first (extension) step of the reaction. Activity values are % of vector control and are means of triplicates; all values differ from vector control with statistical significance (p<0.01).

	Telomerase activity (as % of vector control)*
	** Amplification: ACX reverse primer (specific to wild-type TTAGGG)		** Amplification: 4A10A reverse primer (specific to mutant TTTGGG)
	** Extension: 4 dNTPs	** Extension: dGTP & dTTP	** Extension: 4 dNTPs	** Extension: dGTP & dTTP
Vector Control	100	0	0	5
MT-mTer	100	0	270	270
MT-mTer/siRNA	5	0	350	350

^*All activity values are means of triplicates

^**Extension and amplification steps illustrated in Figure 2D