Figure 1. Type 1 Crys rescue light-dependent magnetoreception in Cry-deficient flies.

a, A tim-GAL4 driven Drosophila transgene (tim-GAL4/UAS-dcry) rescues magnetic responses in cry^b flies, similar to the responses of wild-type Canton-S flies, while tim-GAL4/+ or UAS-dcry/+ alone do not. Bars show preference index values for naïve (white) and trained (black) groups. Numbers represent groups tested. *, P<0.05; ***, P<0.001. Genotypes in parentheses: tim-GAL4/+ (y w; tim-GAL4/+; cry^b), UAS-dcry/+ (y w; UAS-mycdcry/+; cry^b), and tim-GAL4/UAS-dcry (y w; tim-GAL4/UAS-mycdcry; cry^b).

b, A tim-GAL4 driven monarch (dp)cry1 transgene (tim-GAL4/ UAS-dpcry1) rescues magnetic responses in cry^b flies, while tim-GAL4/+ or UAS-dpcry1/+ alone do not. Bars show preference index values for naïve (white) and trained (black) groups. **, P<0.01. Genotypes in parentheses: tim-GAL4/+ (y w; tim-GAL4/+; cry^b), UAS-dpcry1/+ (y w; UAS-mycdpCry1#15b/+; cry^b), and tim-GAL4/ UAS-dpcry1.

c, Irradiance curves for different light conditions. Light measurements were taken from inside the training and test tube. Full-spectrum and >420 nm irradiance curves were reported before¹.

d, Wavelength-dependence of magnetic responses is rescued by monarch (dp)Cry1 (y w; tim-GAL4/UAS-mycdpCry1#15b; cry^b). The full-spectrum data are the same as those depicted in b. Bars show preference index values for naïve (white) and trained (black) groups. *, P<0.05, **, P<0.01.

Values from a, b, and d are mean ± s.e.m.