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. Author manuscript; available in PMC: 2011 Feb 11.
Published in final edited form as: Nature. 2010 Jan 24;463(7282):804. doi: 10.1038/nature08719

Figure 3. Effects of terminal tryptophan mutations on Type 1 and Type 2 Cry-mediated magnetosensitivity.

Figure 3

a, The Drosophila CryW342F mutation rescues magnetosensitive responses. Two mutant lines (111a and 132a) of y w; tim-GAL4/UAS-dcryW342F; cryb were tested. Bars show preference index values for naïve (white) and trained (black) groups. Numbers represent groups tested. *, P<0.05, **, P<0.01.

b, The monarch Cry1W328F mutation rescues magnetosensitive responses. Three mutant lines (126a, 109a, and 18a) of y w; tim-GAL4/UAS-dpcry1W328F; cryb were tested. Bars show preference index values for naïve (white) and trained (black) groups. Numbers represent groups tested. *, P<0.05, **, P<0.01; ***, P<0.001.

c, The monarch Cry2W345F mutation does not rescue magnetosensitive responses. Two mutant lines (130a and 131) of y w; tim-GAL4/ UAS-dpcry2W345F; cryb were tested. Bars show preference index of the naïve (white) and trained (black) groups. Numbers represent groups tested. For a–c, none of the UAS-trp mutant/+ lines (without driver) restored magnetosensitive behavioural responses (data not shown). Values from a–c are mean ± s.e.m. All Cry mutations were sequenced and confirmed from all of the different transgenic lines used.

d, Cry2W345F still inhibits Clock:Cycle-mediated transcription in Schneider 2 cells. The dpPerEp reporter (20 ng) was tested in presence (+) or absence (−) of dpClk/dpCyc expression plasmids (10 ng each); wild-type dpCry2 (20, 50 and 100 ng) or dpCry2W345F (20, 50 and 100 ng) were used10,11. Luciferase activity relative to β-galactosidase activity was computed. Lower, representative western blot of Cry2 (dpCry2 or dpCry2W345F) and tubulin expression. Values are mean ± s.e.m for three independent experiments.