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. 2010 Feb 17;98(4):576–585. doi: 10.1016/j.bpj.2009.08.063

Figure 6.

Figure 6

Static-sample, natural abundance 13C solid-state NMR spectra for oriented PC or PC/PG bicelles with and without pardaxin. (A) Two-dimensional PISEMA spectrum of a PC bicelle with pardaxin. (B–G) One-dimensional slices from two-dimensional PISEMA spectra of PC bicelles with (black) and without (red) pardaxin in bicelles composed of (B–D) 14-O-PC/6-O-PC (molar ratio 3.2:1.0) and (E–G) 14-O-PC/14-O-PG/6-O-PC (molar ratio 2.6:0.6:1,0) lipids. From top (B and E) to bottom (D and G), the slices represent traces through the Cα, C3, and C14 resonances of the lipid molecules, respectively. (H and I) Summary of measured order parameters for 14-O-PC/6-O-PC bicelles (H) and for 14-O-PC/14-O-PG/6-O-PC bicelles (I). The radii of the spheres are inversely proportional to the measured order parameters of atomic sites along the lipid molecule in bicelles with no pardaxin (see text). The order parameters measured both with and without pardaxin are given beside the relevant carbon. The color of the spheres indicate the relative change in measured order parameters upon addition of pardaxin, with red indicating that the order parameter increases; blue that it decreases; and white indicating no change.