Fig. 8.

Characterizaton of the At3g56830 T-DNA insertion. a Five types (I through V) of cDNA of At3g56830 were identified by RACE. White and black bars represent UTRs and coding regions, respectively, and the lines indicate introns. The position of unusual intron border sequences (GC) is indicated. The positions of possible translation start sites in the UTR (uATG) are marked along with their possible lengths of translation, which are proportional to the lengths of the lines. The T-DNA insertion site in SALK_021029 is marked with the inverted triangle. b RT-PCR results with gene-specific primers for At3g56830 and for UBQ10 as a loading control in the homozygous SALK_021029 line compared to Col-0, npq6 and npq7. c Digital video imaging of chlorophyll fluorescence of SALK_021029 and of Col-0, npq6 and npq7. The SALK_021029 line showed a wild-type level of NPQ (red), which was similar to Col-0. In contrast, low NPQ levels in npq6 and npq7 appeared blue