Skip to main content
. 2009 Jul 8;20(3):661–671. doi: 10.1093/cercor/bhp134

Figure 1.

Figure 1.

Primary cortical and hippocampal neurons isolated from E14.5 and E16.5 mouse embryonic brains express Tgfβs and their receptors and are responsive to TGFβ treatment. (A) MLEC/PAI–luciferase assay with conditioned medium from DIV4 cortical and hippocampal cultures. Data as mean ± SEM (n = 3). (B) RT-PCR reveals that cortical and hippocampal neurons express Tgfβ receptors I and II, as well as Tgfβ isoforms 1, 2, and 3. (C) Hippocampal neurons activate Smad proteins upon TGFβ stimulation. Smad1/2/3 immunoreactivity in control untreated cells was localized diffusely in the cytoplasm, whereas TGFβ treatment caused a translocation of Smad proteins in the nucleus. Upper panels: DIV4, lower panels: DIV12. Scale bars = 50 μm.