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. Author manuscript; available in PMC: 2010 Feb 12.
Published in final edited form as: Endocrinology. 2003 Feb;144(2):412–422. doi: 10.1210/en.2002-220769

Fig. 6.

Fig. 6

MKP-1 mRNA is rapidly up-regulated by Dex, whereas MKP-3 is down-regulated. Real-time PCR was used to quantify expression of the dual-specificity phosphatases 3CH134 (mouse MKP-1), CL100 (human MKP-1), and Pyst1 (human MKP-3) in osteoblast cell lines in response to glucocorticoid treatment. A, Example of a standard curve, GAPDH. B, CL100, approximately 10-fold up-regulation following 30 min of Dex treatment. C, 3CH134, approximately 10-fold up-regulation following 30 min of Dex treatment. D, Pyst1, little initial up-regulation and approximately 10-fold down-regulation following 24 h of Dex treatment. Untreated controls (large open circles), treatment with Dex for 30 min (filled circles), 1 h (cross), 4 h (diamond), 8 h (plus), 24 h (filled square). Template concentration was normalized using the housekeeping gene, GAPDH, and fold differences were calculated using the relative standard curve method (see Materials and Methods). Data shown are representative of three independent experiments, and of several duplicate repeats performed on the same batch of cDNA from each experiment (see Table 1).