TABLE 1.
Effects of PMA and GFX on proton channel parameters (mean ± S.E. (n))
Relative values of proton channel parameters in each cell before (c = control) or after PMA stimulation and after GFX reversal are shown. All ratios are presented so that a larger number means a larger effect. The gH-V relationship shift by PMA is relative to control, and GFX is relative to PMA (see “Experimental Procedures”). The T29A, T29D, and double mutant T29A/S97A did not respond. Significance by one-sample t test, *, p < 0.05, **, p < 0.01, compared with no effect (1.0 for ratios, 0 for gH-V shift). None of the differences between WT and S97D or S97A are significant (Student's t test). For comparison, average parameter values from human neutrophils from CGD patients and normal subjects from a previous study (36) are given in columns 4 and 5, respectively.
| WT | S97A or S97D | CGD neutrophils | Neutrophils | |
|---|---|---|---|---|
| IH PMA/c | 2.39* ± 0.51 (10) | 1.42** ± 0.13 (12) | 2.55 | 3.3 |
| IH PMA/GFX | 2.19** ± 0.37 (12) | 1.22 ± 0.11 (8) | ||
| τact c/PMA | 2.12 ± 0.53 (10) | 1.62** ± 0.15 (12) | 1.94 | 3.7 |
| τact GFX/PMA | 1.87** ± 0.22 (12) | 1.51* ± 0.21 (8) | ||
| τtail PMA/c | 1.22 ± 0.11 (10) | 1.13 ± 0.067 (12) | 1.15 | 5.5 |
| τtail PMA/GFX | 1.54** ± 0.17 (13) | 1.14 ± 0.06 (8) | ||
| gH-V shift PMA (mV) | −10.7** ± 1.9 (7) | −7.8** ± 1.6 (8) | −13.3 | −38.8 |
| gH-V shift GFX (mV) | 9.4** ± 1.6 (8) | 6.7** ± 1.2 (7) |