FIGURE 3.

Activities of FGF19dCTD. A, schematic diagram showing FGF19 and FGF19dCTD. B, L6 cells were transfected with FGFR1c or FGFR4 and with β-Klotho. Following overnight serum starvation, cells were stimulated with vehicle, 50 nm recombinant FGF19 or FGF19dCTD for 10 min, and snap frozen in liquid nitrogen. Cell lysates were prepared for Western blot using antibodies against phosphorylated ERK1/2 (p-ERK) or total ERK1/2 (T-ERK). C, semiquantitative analysis of BrdU immunostaining of livers from female FVB mice treated for 6 days with PBS, 2 mg/kg/day recombinant FGF19, or 2 mg/kg/day FGF19dCTD. The scores assigned to BrdU incorporation for these animals were based on a semiquantitative scale described under “Experimental Procedures.” The BrdU immunostaining of livers were showed in supplemental Fig. S2. Solid bars represent the group mean score with S.E. (n = 8 for each group).