FIGURE 5.
Activities of FGF21/1938–42. A, schematic diagram showing FGF21/1938–42. B, L6 cells were transfected with FGFR1c or FGFR4 together with β-Klotho. Following overnight serum starvation, cells were stimulated with vehicle, 50 nm recombinant FGF19, FGF21, or FGF21/1938–42 for 10 min, and snap frozen in liquid nitrogen. Cell lysates were prepared for Western blot using antibodies against phosphorylated ERK1/2 (p-ERK) or total ERK1/2 (T-ERK). C, semiquantitative analysis of BrdU immunostaining of livers from female FVB mice treated for 6 days with PBS, 2 mg/kg/day recombinant FGF19, or 2 mg/kg/day FGF21/1938–42. The scores assigned to BrdU incorporation for these animals were based on a semiquantitative scale described under “Experimental Procedures.” The BrdU immunostaining of livers are shown in supplemental Fig. S5. Solid bars represent the group mean score with S.E. (n = 8 for each group).