TABLE 1.

Kinetic parameters of purified F. tularensis acid phosphatases

	Vmaxa	Kma	kcatb	kcat/Km
	μmol min−1mg−1	mm	s−1	m−1s−1
AcpA	0.25 ± 0.05	0.78 ± 0.18	0.24	3.0 × 102
AcpB	11.84 ± 0.87	3.11 ± 0.28	4.48	1.44 × 103
AcpC	2.03 ± 0.08	0.88 ± 0.13	0.97	1.10 × 103
Hap	0.30 ± 0.00	0.68 ± 0.01	0.23	0.34 × 103

^a Estimated by nonlinear regression analysis using MicroCal Origin 8.

^b k_cat was determined based on the monomeric molecular weight of each protein.