. 2009 Dec 18;285(8):5212–5223. doi: 10.1074/jbc.M109.049833

TABLE 2.

Equilibrium constants for RNA_11F7t binding determined by fluorescence competition

Binding parameters were determined from competition studies similar to those illustrated in Fig. 4 inset. Fitted parameters are listed ±95% confidence limits.

Competitor^a	RNA_11F7t/OG₄₈₈-Xa interaction			RNA_11F7t/competitor
Competitor^a	K_d	Mol RNA/mol OG₄₈₈-Xa	F_max/F_o	K_d	Mol RNA/Mol competitor
	nm			nm
Xa_i	1.26 ± 0.23	1.96 ± 0.04	1.76 ± 0.01	0.61 ± 0.10	2.08 ± 0.04
Prothrombin	ND^b
X	0.79 ± 0.13	2.0 (F)^c	1.75 ± 0.01	0.56 ± 0.08	1.89 ± 0.05
ΔGlaXa_i	0.42 ± 0.13	2.0 (F)	1.70 ± 0.01	0.35 ± 0.10	1.78 ± 0.05
desGlaXa_S195A	1.12 ± 0.40	1.87 ± 0.04	1.75 ± 0.02	0.87 ± 0.28	2.05 ± 0.07
desGlaX_S195A	1.56 ± 0.36	2.09 ± 0.05	1.78 ± 0.01	0.85 ± 0.19	1.88 ± 0.04

^a Fluorescence intensity was measured using increasing concentrations of the indicated competitor in the presence of OG₄₈₈-Xa and two or three different fixed concentrations of RNA_11F7t.

^b No competition detected.

^c Terms used as constants in the analysis are indicated as F.