FIGURE 1.

NFATc1 protein expression is down-regulated during late stage osteoclastogenesis. A–C, BMMs were cultured with M-CSF and RANKL for the indicated times. A, total RNA was collected from each time point and analyzed by Northern blot using probes for c-Fos, NFATc1, OSCAR, and TRAP. B, whole cell extracts were subjected to Western blot analysis with c-Fos, NFATc1, OSCAR, and actin. C, whole cell extract (upper), cytoplasmic (middle), or nuclear (lower) fractions were harvested from cultured cells and analyzed by Western blot for detection of NFATc1. Actin and retinoblastoma-associated protein (Rb) were used as loading controls.