FIGURE 4.

Mapping of hGTSE-1 region involved in p21 binding and stability. A, schematic representation of hGTSE-1 deletions and their in vitro binding with p21. B, U2OS cells were transfected with vectors encoding hGTSE-1-(1–221) and FLAG-p21 and subjected to immunoprecipitation with an anti-FLAG antibody. C, U2OS cells were transfected with vectors encoding hGTSE-1-(1–221) and HA-WISp39 and subjected to immunoprecipitation (IP) with an anti-HA antibody. #, unspecific band. D, U2OS cells were transfected with vectors encoding p21 with an empty vector or with hGTSE-1-(1–221) for 36 h. A vector encoding GFP-tagged histone H2B (GFP-H2B) was used as transfection efficiency control. FL, full-length.