Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Mutant Δ7,8-LDS was produced by homologous recombination using A. tumefaciens-mediated transformation. 2.5 kb of the LDS gene, including putative heme ligands of 7,8-LDS, was replaced with SGFP and TrpC/hph cassettes. The modified strains were characterized by PCR analysis, Southern hybridization, and by analysis of enzymatic activity.
