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. 2009 Dec 20;285(8):5308–5316. doi: 10.1074/jbc.M109.062810

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FIGURE 5. — LC-MS/MS analysis of oxylipins produced by M. oryzae Guy11 (wild type) and the genetically modified strain, Δ7,8-LDS. The top two chromatograms show MS/MS analysis of m/z 311→ m/z 173, a characteristic fragment of 7,8-DiHODE (⁻OOC-(CH₂)₅-CHOH-CHO). This metabolite could not be detected in the mutant Δ7,8-LDS (ion chromatogram magnified 100 times). The bottom two chromatograms show MS/MS analysis of m/z 295 → m/z 157, the characteristic fragment of 8-HODE (⁻OOC-(CH₂)₆-CHO). This metabolite could be detected only in trace amounts in mutant Δ7,8-LDS (ion chromatogram magnified 100 times), and it could possibly be formed by autoxidation.