FIGURE 3.

Nuclear accumulation of p75 in PC12 cells. PC12 cells were treated for 1 h with the nuclear export inhibitor leptomycin B (Lepto B; 20 ng/ml), vehicle control solution (CTRL), or a combination of leptomycin B and γ-secretase inhibitor XVIII (Lepto B + X; 1 μm final concentration) and then fixed and immunostained with anti-p75 antibody. Inhibition of the nuclear export machinery promoted an accumulation of p75 immunoreactivity in the nucleus as shown by co-localization with 4′,6-diamidino-2-phenylindole (DAPI), which is blocked by the inhibition of γ-secretase.