FIGURE 3.

Determination of the mitochondrial cleavage sequence of human AGXT2. A, human AGXT2FLAG was purified from a mitochondrial preparation of AdAGXT2FLAG-infected COS-7 cells by FLAG affinity chromatography, separated by SDS-PAGE, transferred to PVDF membranes, and stained with Coomassie Blue to identify a major mature protein of 52 kDa and a minor precursor protein of 60 kDa. The 52-kDa band was excised from the membrane and subjected to partial N-terminal sequencing. B, N-terminal sequencing results aligned with the predicted N-terminal amino acid sequence of full-length human AGXT2.