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. 2009 Nov 24;285(8):5461–5471. doi: 10.1074/jbc.M109.037127

FIGURE 4.

FIGURE 4.

Decreased cell adhesion in HDF and HTM cells mediated by the down-regulation of ITGB1 by miR-183. pITGB1 or control (pCon) plasmids were co-transfected with either 183M or ConM into HDF (A) and HTM (B) cells. Three days post-transfection, cell adhesion ability was determined using the Vybrant cell adhesion kit (Invitrogen) in plates coated with laminin (1 mg/ml), gelatin (2% solution type B), collagen type I (0.1% solution), or fibronectin (FN, 0.5 mg/ml). Data represent percentage changes compared with control (ConM + pCon) ± S.D. n = 4–6, *, p < 0.05, compared with ConM plus pCon by Mann-Whitney U test. C, cell migration was not affected by miR183 in HTM cells. Assays were performed in HTM cells 3 days post-transfection using the Oris Cell Migration Assay kit. Data represent percentage changes compared with their individual controls (ConM + ConS or ConM + pCon) ± S.D. n = 4–6. Error bars are S.D.