FIGURE 6.

Effects of KIF2A and miR-183 on bipolar spindle formation. A, HeLa, HDF, and HTM cells were transfected with 183M, KIF2A siRNA (KIF2A-S), or their controls (ConM or ConS). One (HeLa) or 3 days (HDF and HTM) post-transfection cells were stained with specific antibodies for KIF2A (red), α-tubulin-FITC (green), and 4′,6-diamidino-2-phenylindole (blue). Immunofluorescence images were recorded using a Nikon C90i confocal automated microscope, and visualized with EZ-C1.3.10 Nikon confocal software. The images presented in panel A are representative results from three independent experiments. B, quantitative data of bipolar cells in HeLa cells. Data represent percentage changes of bipolar cells compared with their individual controls. C, changes in expression of mRNA of aurora kinases AURKA and AURKB measured by real time Q-PCR using specific primers and β-ACTIN was used as internal normalization control. Data represent fold-changes between cells transfected with 183M and ConM ± S.D. (n = 3, *, p < 0.05, Mann-Whitney U test). Error bars are S.D.