FIGURE 1.

JKAP co-localizes with actin filaments and reduces cell migration. A, H1299 cells were stably transfected with GFP (upper panel) or GFP-tagged JKAP (lower panel, Field 1 and Field 2) and stained with TRITC-conjugated phalloidin for F-actin and 4′,6-diamidino-2-phenylindole for nucleus. B and C, H1299-JKAP_TR cells and H1299-JKAP-CS_TR cells were cultured with or without 2 μg/ml tetracycline (Tet) for 15 h. The protein levels and cell migration abilities were examined by Western blotting (left panel) or by Transwell assays (right panel), respectively. EGF, epidermal growth factor; exo, exogenous JKAP; endo, endogenous JKAP. Quantitative data were means ± S.E. of one representative result from three independent experiments. *, p < 0.05; **, p < 0.01, compared with the Tet− group.