FIGURE 2.
Expression of JKAP decreases cell motility. H1299-JKAPTR cells were cultured with or without 2 μg/ml tetracycline (Tet) overnight before the gap-closing assay. A, after a gap was created, cells migrating into the gap region were monitored at 0, 15, and 24 h. Quantitative data were means ± S.E. of one representative result from three independent experiments. **, p < 0.01, compared with the Tet− group. B, migrating H1299-JKAPTR cells that were cultured with 2 μg/ml tetracycline were fixed at 15 h (Field 1 and Field 2) and subjected to double immunostaining with anti-Myc antibody for JKAP-Myc and TRITC-conjugated phalloidin for F-actin. Magnifications of the insets are shown in the right panels.