FIGURE 3.

JKAP decreases FAK phosphorylation in vitro. A, tyrosine-phosphorylated proteins were immunoprecipitated (IP) from pervanadate (5 μm, 1 h)-treated cell extracts and subjected to JKAP phosphatase assay. B and C, cellular FAKs were pulled down with GST-Src-SH2 fusion protein (B) or immunoprecipitated with the anti-FAK antibody (C) as substrates and then left untreated or treated with GST-JKAP-WT or GST-JKAP-CS proteins to perform phosphatase reactions. Equal amounts of reaction mixtures were subjected to Western blot (WB) analyses using specific antibodies as indicated. Results shown are one representative result of three independent experiments.