FIGURE 5.

Knockdown of JKAP expression increases cell migration and FAK phosphorylation. A, levels of indicated proteins and cell migration abilities of H1299-pSUPER, H1299-shJKAP-1, and H1299-shJKAP-2 cells were examined by Western blotting (upper panel) or by Transwell assays (lower panel), respectively. Quantitative data were means ± S.E. of one representative result from three independent experiments. *, p < 0.05; **, p < 0.01, compared with H1299-pSUPER cells. B, H1299-pSUPER and H1299-shJKAP-2 cells were stably transfected with Src. The levels of indicated proteins in cell extracts were examined by Western blot analyses. Results shown are one representative of three independent experiments.