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. 2009 Nov 11;285(8):5532–5540. doi: 10.1074/jbc.M109.074930

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FIGURE 4. — Nuclear localization of ASF/SF2 is increased by IGF-1. A–F, cells were treated with vehicle or IGF in the presence or absence of SRPIN340 and stained for ASF/SF2 and counterstained with Hoechst. A, podocytes show expression of ASF/SF2 in the nucleus and in the cytoplasm. B, IGF induces nuclear localization of cytoplasmic ASF/SF2. C, SRPIN340 by itself does not affect localization of ASF/SF2. D, SRPIN340 inhibited this IGF-mediated localization. E, HEK cells also show cytoplasmic localization of ASF/SF2. F, in contrast, HeLa cells have nuclear ASF/SF2 localization. G, RT-PCR of mRNA from HEK cells shows VEGF₁₆₅b expression, but not in HeLa cells. MWM, molecular weight marker.