FIGURE 6.

Neovascularization induced by hyperoxia is inhibited by a single dose of SRPK inhibitor, SRPIN340. A, low power fluorescence micrograph of FITC-labeled lectin staining of retinal whole mounts with areas of NV (white) and ischemic (orange) outlined. B, higher power view of a single retinal quadrant, with angiogenic areas highlighted by arrowheads. C, high power view of retinal angiogenic area showing sprouting endothelial cells. D, quantification of neovascular areas shows a small but significant inhibition by a single injection of 1 μl of 10 μm SRPIN340 1 day after removal from oxygen. E, ischemic area was also reduced in these mice. F, normal area was consequently increased. G, data shown as relative to control, uninjected contralateral eye. Neo, neovacular; isch, ischemia; Norm, normal.