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. 2009 Dec 18;285(8):5541–5554. doi: 10.1074/jbc.M109.047050

FIGURE 1.

FIGURE 1.

ITPKA controls cell motility. A, whole cell lysates from cells cultivated in complete medium were analyzed for ITPKA expression by Western blot. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression served as loading control. IMR-90, normal lung epithelial cells; A549 and H1299, non-small lung cancer cells; HmEpc, normal breast epithelial cells; T47D, SKBR-3, and MDA231, breast cancer cells; HSF14, normal skin fibroblasts; Mevo, melanoma cells; human leukocytes; Jurkats, leukemic T-cells; HEK293, transformed embryonal kidney cells; HepG2, liver cancer cells. B, ITPKA expression was stably up-regulated in A549 cells and stably down-regulated in MDA231 and H1299 cells. C, cell lines were seeded in wells covered with 15% gelatin solution and monitored by light microscopy after 48 h. One out of three representative experiments is shown for each cell line. D, migration of cells cultivated in complete medium was examined by the transwell assay. Migration (“trans-migrated cells”) was expressed as relative amounts with respect to control cells (considered as 100%). Data represent mean values ± S.D. of four independent experiments. ***, p < 0.0001.