. 2009 Dec 18;285(8):5541–5554. doi: 10.1074/jbc.M109.047050

TABLE 1.

Effect of ITPKA on protrusion size of H299 cells

H1299 cells were transfected with GFP (H1299-GFP), GFP-ITPKA-L34P (H1299-L34P), or with GFP-ITPKA (H1299-ITPKA), and cellular protrusions were counted from at least 100 cells strongly expressing GFP and GFP-ITPKA-L34P. In addition, cell processes were determined from at least 100 H1299 cells expressing low (+), intermediate (++), or high (+++) levels of GFP-ITPKA. The strength of the expression level was determined by the green fluorescence of GFP and GFP-ITPKA fusion proteins. To quantify the protrusions, the same cell was monitored by light and by fluorescence microscopy, and protrusions were only counted from cells monitored by light microscopy.

	% of cells extending at least one protrusion; length >1 μm, width >0.6 μm	No. of protrusions per cell	% of cells extending at least one protrusion; length >4 μm, width >0.6 μm	No. of protrusions per cell
H1299-GFP	44	1.8	2	1
H1299-L34P	51	1.9	0	0
H1299-ITPKA +	36	2.2	1	1
H1299-ITPKA ++	88^a	4.5^a	14^a	1.7^a
H1299-ITPKA +++	95^a	6.9^a	52^a	2.0^a

^a p < 0.0001 and statistically significant differences in protrusion number relative to controls (H1299-GFP) are shown.