TABLE 2.

Effect of ITPKA on relative content of G-actin and F-actin in extracts from H1299 cells

Extracts of H1299 cells stably expressing GFP, GFP-ITPKA, or GFP-ITPKA L34P were examined for G- and F-actin contents by using the DNase I inhibition assay. Values for G-actin and F-actin are % of total actin measured in cell lysates. G-actin was directly measured, and after depolymerizing F-actin with 1.5 m guanidinium chloride, a second DNase I assay was carried out to measure total actin. Data shown were calculated as F-actin = total actin − G-actin. The results are mean values ± S.D. of four experiments.

	G-actin	F-actin
	%	%
H1299-GFP	53.65 ± 1.79	46.35 ± 1.86
H1299-L34P	53.03 ± 3.55	47.00 ± 3.81
H1299-ITPKA	40.97 ± 0.05	59.03 ± 0.04a

^a p < 0.01 and statistically significant differences in F-actin content as compared with controls (H1299-GFP) are shown.