FIGURE 12.

Nuclear localization of ¹²⁵I-ACE is impaired by selective inhibitors of endocytosis. A, content of ¹²⁵I-ACE in nuclei (10⁶) that were isolated from cells incubated with ¹²⁵I-ACE in the absence (control) or presence of endocytosis inhibitors of phenylarsine oxide (PAO), concanavalin A (Con-A), methyl-β-cyclodextrin (M-BCD), and momodansyl cadaverin (MDC), as described under “Experimental Procedures.” ¹²⁵I-ACE in the nuclei of control cells and cells were incubated with the inhibitors at 37 °C (gray bars). ²⁵I-ACE in the nuclei of control cells and cells were incubated with the inhibitors at 4 °C and then transferred to 37 °C after elimination of unbound ²⁵I-ACE (black bars). B, as in A except that the inhibitors tested were amiloride, dextran sulfate, polyinosine, and fucoidan. Error bars are the S.E. ± mean of three determinations (n = 3).