FIGURE 2.

Exogenous ACE triggered a late activation of FAK and SHP2. Analysis of the activation of FAK (125.0 kDa), SHP2 (80.0 kDa), AKT (70.0 kDa), and MAPK (44/42 kDa) was done by monitoring their phosphorylated state as described under “Experimental Procedures.” A, Western blot of total SMC extract, at different times after the addition of serum-free medium (Control) or serum-free medium containing 10 nm ACE, probed with the anti-phosphoprotein antibodies against the proteins indicated on the right-hand side column. The original membrane was cut into three sections, probed with antibodies against the indicated phosphoproteins, recomposed, and developed. Wells between 5 and 60 min, which were left empty, were deleted from the final image. B and C, graphs showing the densitometry plots of the Western blot bands corresponding to the activation of FAK and SHP2, respectively, in the absence and presence of ACE. Error bars are the S.D. ± mean values of eight determinations (n = 3).