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ACE is localized to the nuclei of SMC and HUVEC. A, indirect immunofluorescence images were obtained from SMC, HUVEC, and RFL-6 monolayers incubated with 10 nm TRITC-ACE (red) for 120 min as described under “Experimental Procedures.” TRITC-ACE/DAPI is the merging of TRITC-ACE and DAPI images. Scale bars are at the lower right-hand corners. B, time lapse live cell confocal imaging of TRITC-ACE uptake by SMC. Arrows indicate nuclei that are progressively incorporating TRITC-ACE.
