Skip to main content
. 2009 Dec 14;285(8):5594–5605. doi: 10.1074/jbc.M109.068346

FIGURE 4.

FIGURE 4.

GILZ inhibits FOXO1-WT transcriptional activity in HL-60 cells. A, HL-60 cells were transiently transfected with the reporter plasmid p3xIRS-MLP-Luc and either 1 μg of pcDNA3-FOXO1-WT and/or 5 μg of pcDNA3-Myc-GILZ or pcDNA3-Myc, and transcriptional activity was analyzed after 24 h of culture. Results are expressed as the percentage of p3xIRS-MLP-Luc activity, with 100% representing the activity of the construct in the presence of FOXO1-WT. Data represent the mean ± S.E.M. of three independent experiments performed in triplicate. *, p < 0.05. B, HL-60 cells were transiently transfected with 10 μg of pcDNA3-FOXO1-WT and/or 10 μg of pcDNA3-Myc-GILZ or pcDNA3-Myc and cells were harvested after 24 h of culture. Western blot was performed using anti-FOXO1 and anti-GILZ antibodies. β-Tubulin was used as an internal control of protein levels. IB, immunoblot. Error bars, S.E.M.