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. 2009 Dec 14;285(8):5594–5605. doi: 10.1074/jbc.M109.068346

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FIGURE 5. — GILZ inhibition of FOXO-mediated transcription is not dependent on PKB and IKK phosphorylation sites. A, schematic representation of FOXO1-TM, FOXO3-TM, and FOXO4-TM. HL-60 cells were transiently transfected with reporter plasmid p3xIRS-MLP-Luc, with 5 μg of pcDNA3-Myc-GILZ or pcDNA3-Myc, and/or 0.5 μg of pcDNA3-FOXO3-TM (B), 1 μg of pMT2-FOXO4-TM (C), 0.5 μg of pcDNA3-FOXO1-TM (D), 1 μg of pcDNA3-FOXO3-WT-S644A (E), 0.5 μg of pcDNA3-FOXO3-TM-S644A (F), and transcriptional activity was analyzed after 24 h of culture. Results are expressed as the percentage of p3xIRS-MLP-Luc activity, with 100% representing the activity of the construct in the presence of FOXO-TM. Data represent the mean ± S.E. of three independent experiments performed in triplicate. *, p < 0.05. Error bars, S.E.M.