FIGURE 6.

FOXO3 is mainly cytoplasmic in the presence of GILZ. HL-60 cells transfected with 5 μg of pcDNA3-FOXO3-TM, with or without 10 μg of pcDNA3-Myc-GILZ were harvested 24 h after transfection, and nuclear and cytoplasmic extracts were performed. The 5′-biotinylated IRS probe coupled to streptavidine-agarose beads was incubated with cell extracts. Bound proteins were identified by Western blotting using the specific anti-FOXO3 antibody. A representative experiment of three is shown. IB, immunoblot.