TABLE 1.

Oligonucleotides used for mutagenesis and plasmid constructions

Name	Sequence	Purpose/use
Nde Pi F	GGGAATTCCATATGAGACTCAAGGTCATGATGGAGCTG
Bam Pi R	GGCGGGATCCTTACTCGAGCCCCTTAGCTTTTTTGGGAGG	Amplification of Pi ORF
P106LF106SF	GCTTTAGCTAAAGAGCTTAACCTGCTCTCTACTGCTAAAAACTCC	Generation of P10LF107S mutation in Pi ORF
P106LF106SR	CTCTTCAGGGGAGTTTTTAGCAGTAGAGAGCAGGTTAAGCTCTTT
R75A Pi F	CCCCATCGCTTGCTTATGCACAATTAAAAGAGGGTGGTAAATTAC
R75A Pi R	GTAATTTACCACCCTCTTTTAATTGTGCATAAGCAAGCGATGGGG	Generation of R75A mutation in Pi ORF
D226A Pi F	CCCTGACTTTCCTATTTTTAAAAGGGCTGTGTTAAATAAAGCCATTGCTG
D226A Pi R	CAGCAATGGCTTTATTTAACACAGCCCTTTTAAAAATAGGAAAGTCAGGG	Generation of D226A mutation in Pi ORF
Alpha1	GATCTGATATCAAAATCTGAGGGATTTCGTAGCTGACTGCA	α iteron cloning in looping vector at BglII-Pst site
Alpha2	GTCAGCTACGAAATCCCTCAGATTTTGATATCA
Gamma 1	CTAGTGGGCCCAAACATGAGAGCTTAGTACGTAT	γ iteron cloning in looping vector at Spe-XbaI site
Gamma2	CTAGATACGTACTAAGCTCTCATGTTTGGGCCCA
BglII Gamma F	GATCTCCATGGAAACATGAGAGCTTAGTACGTACTGCA	γ iteron cloning in looping vector at BglII-Pst site
Pst Gamma R	GTACGTACTAAGCTCTCATGTTTCCAGGTA
Complete Gamma F	GGACTAGTCAAAAATTGCTTTGAGAGGCTCTAAGG	Complete γ cloning in looping vector Spe-XbaI site
Xba1 gamma R	GCTCTAGAGATCTGAAGATCGGCAGTTCAACCTG
Alpha MF	GTGCTTCAGTATGTACAGAAATGCAAAACATGAGAGCTTAGTACGCTGAAAGATCGCCAGTCTTCGACCG	Replacement of α iteron by γ iteron
Alpha MR	CCTTACGGTCGAAGACTGGCGATCTTTCAGCGTACTAAGCTCTCATGTTTTGCATTTCTGTACATACTGAAG
NotPiF	GAGCGGCCGCACGTTAAAACATGAGATAAAAATTG	Amplification of Pi ORF with its own promoter
PstPiR	AACTGCAGTCAAGCTTCATCATCTTTATCGCCAG
KanF	GCGGGAATTCGGGTCTCCGCAAGTGGCACTTTTCGGGGAAATG	Cloning of Kanamycin gene in pUC Kn vector
KanR	GCGGGAATTCAGCGTCGATTTTTGTGATGCTCGTCAGGGG
Alpha350	GCCACAACTGAAATTGTGCTTCAG	Used to detect α iteron in Chip assay
Alpha475	CGAACGCCCCGCACAGGTCTTACAG
Gamma373	CCTTAGAGGCTATTTAAGTTGCTG	Used to detect γ iteron in Chip assay
Gamma R	AGTACGTACTAAGCTCTCATGTTTCAC