FIGURE 3.

SS to DS replication in vitro of DNA templates carrying the wt R6K G site, the origin of G4 (M13 G ori1), and the two mutant forms of the plasmid G site. A, sequences showing the wt and the Not1 and the CAG mutant forms; the bottom line shows the start point of the presumptive primer RNA. B, diagram showing the purified proteins used and their action in replicating SS to DS DNA. C, quantification of the reaction product from each template from three separate experiments, presented with S.E. bars. D, in vivo measurements of maintenance of amp-resistant IHF⁺ and ihfΔ hosts carrying the wt and the various G site mutants along with a pUC19 control plated on LB media containing 40 μg/ml ampicillin.