TABLE 1.

List of primers

Name of the primer	Purpose/use
GKNOCKF and GKNOCKR	To introduce NotI site
CTGMUTF and CTGMUTR	To mutate primase recognition sequence
IHFMUTF and IHFMUTR	To mutate IHF site in γ origin
dna2XhoF and dna2XhoF	To introduce XhoI site after VIIth iteron
Alfa5F and Alfa5R	To introduce 5-bp insertion between α iteron and G site
Alfa10F and Alfa10R	To introduce 10-bp insertion between α iteron and G site
Alpha F and Alpha R	To amplify α-γ fragment
Amp F and Amp R	To amplify pUC18 ampicillin gene