FIGURE 9.

Selected mass range comparison between the MALDI-TOF MS profiles of the permethylated N-glycans derived from CHO cells grown in suspension (A), the LEC11 mutant cell line (B), the LEC12 mutant cell line (C), and the LEC30 mutant cell line (D). Only structures bearing multiple fucoses in the mutant samples are annotated with structures to simplify the presentation, although all other glycan peaks are labeled with their m/z values alone. For complete annotation of the spectra, see supplemental Table S1. Data were obtained from the 50% MeCN fraction, and all molecular ions are present in sodiated form ([M + Na]⁺).